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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Heart Lung Transplant. 2012 Nov 9;32(1):98–105. doi: 10.1016/j.healun.2012.09.021

Table 2.

Inhibition of Conventional IgG Reactivity in Class I Alleles Exhibiting High C1q Reactivity Is Reversed by Serum Dilution or Heat Treatment

Patient group and ID (number of Class I HLA alleles) Mean fluorescent intensity (MFI, mean ± SD)
p-valuea
1:8 dilution Luminex IgG Luminex IgG 1:8 dilutiona Luminex C1q
4p (12) 4,871 ± 2,733 19,790 ± 1,141 20,780 ± 1,307 <0.001
6p (11) 8,800 ± 2,680 18,135 ± 625 21,601 ± 203 <0.001

Heat treatment Luminex IgG Luminex IgG heat treateda Luminex C1q p-valuea
12a (7) 3,000 ± 970 18,218 ± 1,022 15,920 ± 2,580 <0.001
13a (6) 2,500 ± 1,176 20,438 ± 728 19,876 ± 2,294 <0.001
14a (10) 10,361 ± 3,233 19,745 ± 1,222 21,482 ± 1,659 <0.001
a

Paired t-test: undiluted conventional IgG MFI vs 1:8 serum dilution or heat-treated sera conventional IgG MFI.