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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: J Proteome Res. 2011 Dec 1;11(2):1108–1117. doi: 10.1021/pr200834f

Figure 1.

Figure 1

Transient oxygen/glucose deprivation (OGD) activates SUMO2/3 conjugation and induces cell death. (A) B35 cells were exposed to 6 h or 8 h of OGD and 22 h of recovery. Cell death was evaluated by measuring the release of LDH from cells and relating LDH released from cells to total LDH activity. Data are presented as means ± SD (n=3). Statistically significant differences between groups were evaluated by ANOVA followed by Fisher's PLSD test; *** p≤0.001. (B) B35 neuroblastoma cells were exposed to 6 h of OGD and 0–180 min of recovery. Proteins were extracted as described in Experimental Section, and OGD-induced changes in levels of SUMO2/3 conjugated proteins were evaluated by Western blotting. Free SUMO2/3 is indicated by arrowhead.