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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: J Proteome Res. 2011 Dec 1;11(2):1108–1117. doi: 10.1021/pr200834f

Figure 4.

Figure 4

Verification of proteomic analysis. Proteomic analysis data were verified for RUNX1 (A) and TIF1β (B). HA-tagged SUMO3 conjugated proteins of extracts from control and OGD cultures were immunoprecipitated using anti-HA agarose and eluted with 100 μg/ml HA peptide. Eluates were left intact or incubated with SUMO protease to release SUMO from target proteins, loaded onto SDS-PAGE gels and immunoblotted using antibodies against RUNX1 (A) and TIF1β (B). WB, Western blotting; IP, immunoprecipitation.