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Protein ubiquitination is increased after OGD and depends on SUMO expression. (A) Immunoprecipitates from control and OGD extracts were immunoblotted for ubiqutin, and SUMO protease was utilized to demonstrate SUMO conjugation as in Fig. 4. (B) SUMO2/3 and (C) ubiquitin levels in B35 cells stably expressing control or SUMO2/3 miRNA were analyzed by Western blotting in untreated cells and immediately following or 30 min after 6 h OGD. WB, Western blotting. IP, immunoprecipitation.
