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. 2013 Apr 16;8(4):e60907. doi: 10.1371/journal.pone.0060907

Figure 6. ERK2 is not required for ES cells to efficiently produce neurons under defined conditions.

Figure 6

(A) Immunocytochemistry analysis of wild-type, Erk2 +/− and Erk2 −/− cultures after 5 days of neural induction for neuronal marker βIII Tubulin immunoreactivity (red). DNA is counterstained with DAPI in blue. (B) Gene expression analysis of cultures following 5 and 10 days of neural induction. Markers of pluripotency (Oct4, Nanog, and Rex1) and neural tissue (NeuroD3, Ngn2, and Mash1) are shown. Error bars represent the standard deviation from the mean of biological replicates.