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. 2013 Apr 16;8(4):e62046. doi: 10.1371/journal.pone.0062046

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© 2013 Duncan, Fritzsch

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The top panel, using qRT-PCR, shows the relative level of recombined Gata3 exon4 in the inner ear of both Cre lines compared to control (set to 1, for each age group). There is an earlier and more effective recombination of the Gata3 floxed allele in Foxg1^Cre ears than in Pax2-Cre. Pax2-Cre ears first show a significant reduction in Gata3 exon4 at E10.5 two days after Foxg1^Cre. This is correlated with the timing of sensory vs non-sensory specification in the ear. The lower panel shows a comparison of Cre expression levels, using qRT-PCR, between Foxg1^Cre: Gata3^f/f and Pax2-Cre:Gata3^f/f ears. Cre expression levels are relative to the level of Cre mRNA in Foxg1^Cre: Gata3f/f ears at E8.5 (set to 1). The scale on the left is logarithmic. Pax2-Cre mRNA expression levels are 8-fold less at E8.5 than Foxg1^Cre. All data represent 3 biological replicates and 3 technical replicates per-time point as per MIQE guidelines [50]. *p<.01, students t-test.