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. 2013 Apr 16;8(4):e61962. doi: 10.1371/journal.pone.0061962

Figure 6. Antigen-specific systemic antibodies in serum.

Figure 6

(A) MOMP-specific serum IgG, IgA IgG1 and IgG2a following vaccination was quantified by direct ELISA. Endpoint tires were calculated for all samples using background absorbance of PBST plus two SD. The ratio of IgG2a:IgG1, used to determine Th1:Th2 polarization, is indicated above the titer bars in each group. (B) Percentage of infection neutralized in vitro was determined by incubation of Chlamydia with a 1/10 dilution of whole serum. Results are presented as the mean ± SD. Significant differences were determined using a one-way ANOVA with Tukey’s post-test. Significance was set at P<0.05 for all tests. P>0.05 (not shown), 0.01–0.05 (*), 0.001–0.01 (**) and <0.001 (***).