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. 2013 Apr 16;8(4):e61990. doi: 10.1371/journal.pone.0061990

Figure 5. Decreased odor-evoked OSNs activity leads to an elevated level of TRPM5 mRNA.

Figure 5

The mRNA levels of TRPM5, OMP, and Gαolf were compared between: A. left (open) and right (closed, occluded) OE of the naris-occluded TRPM5-GFP animals. B. CNGA2 knockout/TRPM5-GFP and TRPM5-GFP control OE. mRNA levels are normalized to levels of 18 S rRNA. In the occluded OE, there was significantly higher expression of mRNA of TRPM5 (p = 0.024, n = 10), OMP (p = 0.008, n = 10), and Gαolf (p = 0.033, n = 10, paired t-test, p value FDR corrected p = 0.05).Untreated control animals showed no significant difference between left and right OE (p>0.05, paired t-test, n = 5, data not shown). The mRNA expression level of TRPM5 was significantly higher in CNGA2 knockout/TRPM5-GFP compared to TRPM5-GFP (p = 0.016, unpaired t-test, p value FDR corrected p = 0.0167, n = 5). There was no significant difference between CNGA2 knockout/TRPM5-GFP and TRPM5-GFP in mRNA expression levels for OMP and Gαolf (p>0.05, unpaired t-test FDR corrected, n = 5). Error bars are SEM.