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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Otol Neurotol. 2013 Apr;34(3):559–569. doi: 10.1097/MAO.0b013e3182868312

Figure 4.

Figure 4

qPCR was used to quantify the relative difference in caspase-3, -8 and -9 mRNA between control (+/Y) and mutant (Phex Hyp-Duk/Y) mice after normalizing to GAPDH. (A) shows the fold difference in caspase-3, -8 and -9 between the mutants and controls at P21. A trend towards increased expression of caspase-3 and -9 was present (P=0.1), and a higher expression of caspase-8 (P=0.03) was seen in the mutants compared to controls. (B) At P40, the relative expression of caspase-3, -8 and -9 was significantly higher in the mutants compared to controls (P=0.008, 0.001 and 0.003, respectively). Caspase-8 expression is 6 fold higher in the mutants. Error bars were generated by calculating the Standard Error of the Mean (SEM).