Skip to main content
. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: J Immunol. 2008 Jun 1;180(11):7212–7220. doi: 10.4049/jimmunol.180.11.7212

FIGURE 3.

FIGURE 3

Potentiation of Ag-induced degranulation by NECA in WT and A2BKO mast cells. A, Degranulation of WT and A2BKO BMMCs was assessed by measuring β-hexosaminidase release from cells preincubated with anti-DNP IgE after stimulation with 10 ng/ml DNP-HSA Ag (DNP-HSA, DNP-HSA+NECA) or from nonstimulated cells (Basal, NECA) in the absence (Basal, DNP-HSA) or presence (NECA, DNP-HSA+NECA) of 10 μM NECA. Values are expressed as mean percentage ± SEM of three separate cell preparations. B, Potentiation of Ag-induced degranulation by NECA in WT and A2BKO BMMCs calculated as fold value from data presented in A.