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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: J Immunol. 2008 Jun 1;180(11):7212–7220. doi: 10.4049/jimmunol.180.11.7212

FIGURE 5.

FIGURE 5

Effects of DPCPX and MRS1706 on IL-13 secretion and β-hexosaminidase release from mast cells. A, BMMCs were incubated in the absence (Basal) or presence of 10 μM NECA and in the presence of 1 μM DPCPX, 100 nM MRS1706, or their vehicle for 6 h. IL-13 was measured in culture medium and expressed as picograms per 106 cells. Data are presented as mean ± SEM of five separate cell preparations. **, p < 0.01, significant difference from vehicle determined by one-way ANOVA with Dunnett’s post-test. B, Degranulation of BMMCs was assessed by measuring β-hexosaminidase release from cells preincubated with anti-DNP IgE after stimulation with 10 ng/ml DNP-HSA Ag or from nonstimulated cells (Basal) in the presence of 1 μM DPCPX, 100 nM MRS1706, or their vehicle. Values are expressed as mean ± SEM of three separate cell preparations.