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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: Biotechnol Appl Biochem. 2012 Feb 23;59(2):88–96. doi: 10.1002/bab.1003

Figure 1. 3-D confinement affects growth kinetics of hESCs.

Figure 1

hESCs were cultured in 3-D in 120 μm deep microwells or on 2-D substrates for 6 days. Starting at 14 hours, cells were harvested and counted every 24 hours. Total cell number was normalized to initial cell number and plotted at each timepoint. hESCs cultured in larger microwells (300 and 500 μm/side) exhibited a reduction in cell growth after 82 hours in culture, while this effect occurred earlier in the smallest microwell size (100 μm/side).