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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: Biotechnol Appl Biochem. 2012 Feb 23;59(2):88–96. doi: 10.1002/bab.1003

Figure 2. hESCs cultured in microwells exhibit reduced proliferative capacity.

Figure 2

Every 24 hours, cells on 2-D controls (Matrigel-coated TCPS) and in 100, 300, and 500 μm/side microwells were pulsed with bromodeoxiuridine (BrdU) for 20 minutes. After BrdU treatment, the cells were harvested and the percentage of BrdU-positive cells was determined using flow cytometry. Microwell-cultured cells contained a lower percentage of BrdU-positive cells than 2-D controls, indicating a reduction in proliferative capacity (* indicates P<0.05 compared to same-day 2-D control).