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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: J Pathol. 2011 May 5;224(3):401–410. doi: 10.1002/path.2888

Figure 4.

Figure 4

Liver biopsies of NASH demonstrating Shh positive ballooned hepatocytes which are surrounded by gli-2 positive myofibroblasts. (A) Masson trichrome stained section showing ballooned hepatocytes (arrows) encircled by dense fibrous matrix; vimentin and α-SMA immunohistochemistry highlighting stromal cells positive for these myofibroblastic markers with adjacent ballooned hepatocytes (arrows); and gli-2 immunohistochemistry showing positive nuclear staining in myofibroblasts with adjacent ballooned hepatocytes (arrows). (B and C) The number of Shh positive hepatocytes were counted in each biopsy specimen and normalized to biopsy length. The normalized number of Shh positive hepatocytes were plotted against fibrosis stage (B) and degree of ballooning (C). The mean ±SD results are graphed (*p<0.05, **p<0.001). 40x magnification for all images.