Fig. 4.
Effect of AGS3 expression on heterologous sensitization of AC isoforms. a. HEK293 cells stably expressing AC1 and D2LDR were stimulated with vehicle or quinpirole (1 μM) for 2 hr, washed, and subsequently stimulated with A23187 (10 μM) as indicated. Data represent mean ± S.E.M. of three independent experiments. * p < 0.05, unpaired t-test. b. HEK293 cells stably expressing AC2 and D2LDR in the absence of, or coexpressed with AGS3-Venus or AGS3-Q/A-Venus as indicated were pretreated with vehicle or quinpirole (1 μM) for 2 hr, washed, and subsequently stimulated with PMA (1 μM). Data represent mean ± S.E.M. of at least three independent experiments. ** p < 0.01, one way analysis of variance followed by Dunnett's post hoc test