Table 1.
Data collection and refinement statistics
| Data collection | |
| Beamline | 9.1, SSRL, Menlo Park, CA |
| Wavelength (Å) | 0.979 |
| Space group | C2 |
| Cell dimensions | |
| a, b, c (Å) | 61.452, 106.139, 124.139 |
| α, β, γ (°) | 90.0, 95.08, 90.0 |
| No. of molecules in the asymmetric unit | 3 |
| Resolution range (Å) | 50.0–3.0 |
| Mosaicity range (°) | 0.34–1.15 |
| Total observations | 53,674 |
| Unique reflections | 15,545 |
| Redundancy | 3.5 (2.9) |
| Completeness (%) | 97.5 (92.1) |
| Rmerge (%) | 16.2 (49.1) |
| Average I/σ | 9.43 (2.34) |
| Data processing program | HKL2000 |
| Refinement | |
| Refinement programs | CNS, Refmac5 |
| Resolution range (Å) | 50.0–3.0 |
| R-factor (%) | 23.5 |
| Rfree (%) | 28.8 |
| Solvent content (%) | 54.8 |
| No. of atoms, average B (Å2) | |
| Protein | 5241, 47.2 |
| Water | 46, 24.1 |
| Retinal | 60, 34.5 |
| Lipid | 325, 55.6 |
| RMSD from ideal bond lengths (Å) | 0.010 |
| RMSD from ideal bond angles (°) | 1.55 |
| Ramachandran plot (preferred/allowed/outliers) (%) | 94.0/6.0/0.0 |