Table 2.
Activity of purified MtBgl3a on polysaccharide substrates. Activity was measured, as described in “Materials and methods” section. Activity not detected for substrates p-α-NPG, p-α-NPGal, wheat arabinoxylan, CMC, Avicel, filter paper and birchwood xylan. The experiment was carried out in triplicates.
| Substrate | Specific activity |
|---|---|
| (U/mg protein) | |
| p-β-NPG (1 mM) | 97.7 ± 1.07 |
| p-β-NPGal (5 mM) | traces |
| p-NPCell (1 mM) | 15.9 ± 0.07 |
| cellobiose (10 mM) | 30.7 ± 0.97 |
| laminarin (0.5%) | 52.0 ± 2.20 |
| lichenan (1%) | 20.6 ± 0.04 |
| barley β-glucan (1%) | 12.4 ± 0.18 |