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. 2013 Apr 16;8(4):e59542. doi: 10.1371/journal.pone.0059542

Table 1. Summary of assembly process of 1AL BAC clone fingerprints.

Physical map construction No. of contigs No. of singletons Assembly length (kb)1 N50 (kb)1
Automated assembly Initial build of all fingerprints (cutoff 1e-75) 5737 30990 865109 -
Add singletons & merge (cutoff 1e-70) 4991 28801 801774 -
Add singletons & merge (cutoff 1e-65) 4264 26657 742893 -
Add singletons & merge (cutoff 1e-60) 3662 24824 695190 -
Add singletons & merge (cutoff 1e-55) 3272 24413 664550 -
Add singletons & merge (cutoff 1e-50) 2810 22068 636733 -
Final build before manual editing (cutoff 1e-45) 2551 20807 622795 277
Manual marker and small overlap-based merging of contigs (cutoff 1e-25) 2210 20867 596581 341
After removal of small contigs2 1180 26193 446042 460
1

Calculated based on an average consensus band length of 1.2 kb; does not include singletons.

2

Defined as comprising <6 BAC clones and/or <200 kb in length, with no markers. Clones from these contigs are added to the list of singletons at this stage.