Table 1. Summary of assembly process of 1AL BAC clone fingerprints.
Physical map construction | No. of contigs | No. of singletons | Assembly length (kb)1 | N50 (kb)1 | |
Automated assembly | Initial build of all fingerprints (cutoff 1e-75) | 5737 | 30990 | 865109 | - |
Add singletons & merge (cutoff 1e-70) | 4991 | 28801 | 801774 | - | |
Add singletons & merge (cutoff 1e-65) | 4264 | 26657 | 742893 | - | |
Add singletons & merge (cutoff 1e-60) | 3662 | 24824 | 695190 | - | |
Add singletons & merge (cutoff 1e-55) | 3272 | 24413 | 664550 | - | |
Add singletons & merge (cutoff 1e-50) | 2810 | 22068 | 636733 | - | |
Final build before manual editing (cutoff 1e-45) | 2551 | 20807 | 622795 | 277 | |
Manual marker and small overlap-based merging of contigs (cutoff 1e-25) | 2210 | 20867 | 596581 | 341 | |
After removal of small contigs2 | 1180 | 26193 | 446042 | 460 |
Calculated based on an average consensus band length of 1.2 kb; does not include singletons.
Defined as comprising <6 BAC clones and/or <200 kb in length, with no markers. Clones from these contigs are added to the list of singletons at this stage.