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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Immunol Res. 2013 May;56(1):1–8. doi: 10.1007/s12026-012-8382-7

Figure 3.

Figure 3

Intravenous transfer of bone-marrow-derived DCs modulates protein expression of ligands of co-stimulatory molecules on MOG-primed CD4+ T cells. Splenocytes were isolated from EAE mice treated with i.v. PBS, DCs pulsed with MOG peptide (0.1 μM) (DC-MOG) or not (DC).Cells were stained and CD4+ T cells were gated. Protein expression of OX40, CD152, CD80, CD154, PD-1, CD28, ICOS and BTLA on MOG-stimulated CD4+ T cells derived from mice treated with DC-MOG (thin line), DC without MOG peptide treatment (DC, dot line) and PBS (thick line) is shown. Isotype controls (shade) are also indicated. The error bars shown in this figure represent the mean and SD of triplicate determinations of mean fluorescence intensity (MFI) of co-stimulatory molecule ligands expressed on CD4+ T cells in three independent experiments (n=3, t test, * P<0.05).