Skip to main content
. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2013 Jan 15;97(9):3893–3900. doi: 10.1007/s00253-012-4682-z

Figure 1.

Figure 1

E. coli 6-OST-1 shake flask growth in different media. A) 26 h growth curves on (◊) induced LB; (♦) not induced LB; (□) induced glycerol and soya peptone; (■) not induced glycerol and soya peptone. Induction was performed with 0.2 mM IPTG at 0.6 Abs600nm, as indicated by the arrow. B) SDS-PAGE analysis of crude extracts; lane 1: 250-10 KDa ladder; lane 2: not induced LB, lane 3: induced LB, lane 4: not induced glycerol and soya peptone, lane 5: induced glycerol + soya peptone. 6-OST-1 bands are indicated in lane 3 and 5 by arrows.