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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2013 Jan 15;97(9):3893–3900. doi: 10.1007/s00253-012-4682-z

Figure 2.

Figure 2

SDS-PAGE analysis of 6-OST-1 enzyme obtained by extraction and purification of biomass crude extract after shake flask growth in glycerol and soya peptone; lane 1: 250-10 KDa ladder as reported in Fig. 1, lane 2: purified 6-OST-1 enzyme (indicated by arrow), lane 3: crude extract with 6-OST-1 enzyme (indicated by arrow). The identity of the band at 75 KDa in lane 2 was confirmed by western blot analysis (Data not shown).