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. Author manuscript; available in PMC: 2014 May 1.

Published in final edited form as: Appl Microbiol Biotechnol. 2013 Jan 15;97(9):3893–3900. doi: 10.1007/s00253-012-4682-z

E. coli 6-OST-1 shake flask growth in glycerol + soya peptone medium using different inducers. A) SDS-PAGE analysis of crude extracts; lane 1:IPTG; lane 2: IPTG and Gal, lane 3: Gal LB, lane 4: 250-10 KDa ladder as reported in Fig.1. B) 26 h growth curves induced with (□) IPTG, () IPTG and Gal; (■) Gal. Induction was performed with 0.2 mM IPTG at 0.6 Abs_600nm, as indicated by the arrow.

Inline graphic — E. coli 6-OST-1 shake flask growth in glycerol + soya peptone medium using different inducers. A) SDS-PAGE analysis of crude extracts; lane 1:IPTG; lane 2: IPTG and Gal, lane 3: Gal LB, lane 4: 250-10 KDa ladder as reported in Fig.1. B) 26 h growth curves induced with (□) IPTG, () IPTG and Gal; (■) Gal. Induction was performed with 0.2 mM IPTG at 0.6 Abs_600nm, as indicated by the arrow.