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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mamm Genome. 2013 Feb 13;24(0):95–104. doi: 10.1007/s00335-013-9447-1

Figure 6. Sequence and western blot analysis of whole eye and other organs from SS/Jr and SS/Jr-Ctr.

Figure 6

(A) DNA sequencing chromatographs shows a point mutation in the start codon (ATG → GTG) in SS/Jr-Ctr and F1 DNA compared to wild-type SS/Jr. The start codon (ATG) which encodes a methionine is predicated to be a valine in the SS/Jr-Ctr and F1. (B) Western blot analysis of eye. No CRYGD protein is evident in the SS/Jr-Ctr eye, but is observed in wild-type SS/Jr animals. In eyes from F1 animals, there is approximately 50% reduction in the amount of CRYGD protein observed. (C) Western blot analysis of homogenates from various organs and eye. No CRYGD protein is evident in tissues related to cardiovascular health and disease (heart or kidney).