. Author manuscript; available in PMC: 2014 Apr 1.

Published in final edited form as: Mamm Genome. 2013 Feb 13;24(0):95–104. doi: 10.1007/s00335-013-9447-1

Table I.

Genomic DNA primers used to amplify γ-Crystallin gene family (Crygb-Crygf)

Primer Name	Forward Sequence (5′-3′)	Reverse Sequence (5′-3′)
Crygb Exon1–2	TGTGTGATTTCCTGTGGAGG	TGCTCTAATTGAAACGACTTGG
Crygb Exon3	GAGGTGTCGGGACAGGAAAC	GGAAGTTCCTTCCCATCTCTG
Crygc Exon1–2	TCCGCTAACACAGCAACAAC	GACAAACAAACCCTATTCTCTGG
Crygc Exon3	CGTTGTTTAGCTTGGGTGAG	TGTAGGGAGAGTTTACAGGTGG
Crygd Exon1–2	CCTTTTGTGCTGTTCCTGC	GGCAACTGCAGTCAGGGTC
Crygd Exon3	ATGGCTTGATGCAGCTGAG	CCAATAAACATGAGCAAACAGG
Cryge Exon1–2	TTGTGCTGTTCCTGCCAAC	CGGCCACAGATGTTACTAGC
Cryge Exon3	TGGGTTTCCAAGTTCAGGTC	CATCCTTTCTTTGCTGTGCC

All Crygb-e genes consist of three exons. The first primer pair was used to amplify exon 1–2 (including intron 1) and the second primer pair was used to amplify exon 3.