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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Neurobiol Dis. 2013 Jan 11;54:264–279. doi: 10.1016/j.nbd.2012.12.018

Figure 2. Protection against 6-OHDA-induced cell death in PC12 cells is dependent upon NF-κB up-regulation of antioxidants and anti-apoptotic factors.

Figure 2

PC12 cells were transfected with empty pAAV vector (control), shScramble, shRNF11, or V5-RNF11 pAAV constructs and exposed to saline (vehicle), 20 µM Bay11-7085, or 10 ng/ml TNF-α for 1 hour followed by saline or 100 µM 6-OHDA for 18 hours. Total RNA was extracted and examined by qRT-PCR for expression of the p50 subunit of NF-κB (A), TNF-α (B), GSS (C), SOD1 (D), BDNF (E), BCL2 (F), and RNF11 (G) mRNA. Values expressed are mean comparative cycle threshold ± SEM with GAPDH levels as an internal control. Results were analyzed by two-way ANOVA with Bonferroni post-tests. a, P < 0.001 compared to vehicle treated shRNF11 cells, b, P < 0.001 compared to vehicle treated control cells, c, P < 0.001 compared to vehicle treated shScramble cells, d, P < 0.001 compared to vehicle pretreated and 6-OHDA treated control cells, e, P < 0.001 compared to vehicle pretreated and 6-OHDA treated shScramble cells, f, P < 0.001 compared to vehicle pretreated and 6-OHDA treated shRNF11 cells, g, P < 0.01 compared to vehicle pretreated and 6-OHDA treated shRNF11 cells, h, P < 0.01 compared to vehicle treated control cells, i, P < 0.01 compared to vehicle treated shScramble cells, j, P < 0.001 compared to vehicle pretreated and 6-OHDA treated V5-RNF11 cells, k, P < 0.001 compared to control cells with the same pretreatment and treatment conditions. Bars without letters denoting significance are not significantly different from vehicle treated cells with the same transfected plasmid (Bay 11, TNF-α, and vehicle pretreatment and 6-OHDA treatment conditions) or from vehicle pretreated and 6-OHDA treated cells with the same transfected plasmid (Bay 11 or TNF-α pretreatment and 6-OHDA treatment conditions).