Figure 6. Nogo-B does not affect the mitogenic effect of epidermal growth factor (EGF) in vitro.
Twenty-four hours after the incubation with supplement-free HMM, WT and Nogo-B KO hepatocytes were treated with EGF (20ng/ml) for 48 hours. The number of proliferating cell nuclear antigen (PCNA; 1:3000) positive nuclei (green) was counted and expressed as % PCNA positive nuclei over the total nuclei determined by DAPI staining (blue). * p<0.05 vs control. N=3.
