Figure 3.

Use of probe 2g to detect oncogenic Hsp90 by flow cytometry (a) and (b) and by microscopy (c). For (b) and (c) cells were permeabilized with digitonin. DMSO, cells treated with vehicle only; control, cells treated with vehicle and stained with antibiotin-PE for flow cytometry and antibiotin-FITC for fluorescent microscopy; 2g, cells treated with probe 2g and stained with antibiotin-PE for flow cytometry and antibiotin-FITC for fluorescent microscopy. MFI, mean fluorescence intensity. CD45 is a plasma-membrane protein. For microscopy, nuclei were stained with DAPI. (a) and (b) right panels; quantification of repeat experiments (n = 2).