Table 2.

Primers used in this study.

Name	Sequence (5′ to 3′): Restriction sites underlined	Purpose
Fbre5F	ATATCAGTTGTTGCTCCTAGGGC	5′ Flank ΔcclA
Fbre5R-EcoRI	CATGTGGAATTCAATAACGGTTCACGAGTAAATTG	5′ Flank ΔcclA
Fbre3F-HindIII	TGGGGTAAGCTTGTTCCGAGCCATATCTGTC	3′ Flank ΔcclA
Fbre3R	ACAACAAAACTAGCTCTCTCGGC	3′ Flank ΔcclA
FbreCOMF-NotI	TTGCTCCAGCGGCCGCATCCTCAAACCTCGCCAAGTATG	cclA complementation
FbreCOMR-SpeI	GGAAATACTAGTGAAGCTGAAAGTGACGTCTAGAA	cclA complementation
JP1	TATAGGGACGAATTCACAGACAATG	5′ Flank ΔhdmA
JP2	CGCTGAATTCGGGTTTGATGGACATTGGAC	5′ Flank ΔhdmA
JP3	CCGGTCTAGAGGTAATGCTTAGACTCCCGTA	3′ Flank ΔhdmA
JP4	GTCATGCGGCCGCCACTGCCCTCGTTAAGG	3′ Flank ΔhdmA
JP lsdA comp For	GCTTGGATCCGACGTAGCAGGTGAAC	hdmA complementation
JP lsdA comp Rev	CATTGGATCCTCCTCGCCTTTCTCC	hdmA complementation
JP Afumi argB For	GAACGCGGTCTGCATCCAAG	Af-argB cloning
JP Afumi argB Rev	GAAGGAGAGACCCATACATCC	Af-argB cloning
FumlaeAF	ATGTTTCTCAACGGGCAGGGC	laeA probe
FumlaeAR	ATTGGCGAGAGGTTTTCGAGCC	laeA probe
FbreIF	CTTTCCACACATCAAGTACCGGC	cclA probe
FbreIR	ATTGAAGCGTTCGCCAATGCC	cclA probe
JP LSD1	TCTCACGCAACTACATACGTCAAC	hdmA probe
JP LSD2	TTTTTCCTCTTCGCTGGCTTGCC	hdmA probe
GZINTF	AAGGGCCGGTAGTCTACCTCTTC	gliZ probe
GZINTF	CGATCTGGTAGCTGCCCAGCTGGAAG	gliZ probe