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. 2013 Apr 16;8(4):e61775. doi: 10.1371/journal.pone.0061775

Figure 2. NP protein delivered with alum provides optimal protection from influenza A infection.

Figure 2

B6 mice were immunized i.m. in both hind-legs with PBS (closed triangles) or a total of 10 µg of NP protein delivered alone (closed diamonds), or with 10 µg of MPL (closed circles), 100 µg alum (closed squares), or both adjuvants (open squares). At least 70 days later, these animals were infected with PR8 influenza A i.n. Mice were weighed daily and the percent of original weight calculated (A) or the amount of virus present in one lung lobe examined 4–5 days after infection (B). Data are combined from two separate experiments with 4–5 mice per group. In A, significant differences between the PBS control mice infected with PR8 and the experimental groups are indicated by the following symbol on the indicated days post-infection. Significant differences in mice immunized with NP and alum are indicated by **(p<0.01) and ***(p<0.001). Significant difference in mice immunized with NP and MPL are indicated by ## (p<0.01). Significant differences in mice immunized with NP and alum and MPL are indicated by $ (p<0.05), $$ (p<0.01), $$$ (p<0.001). No significant difference between control PBS mice and those immunized with NP protein were found. In B, * = p<0.05.