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. 2013 Apr 16;8(4):e61754. doi: 10.1371/journal.pone.0061754

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© 2013 Hao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) α-SMA protein assay by immunofluorescence imaging on fibroblasts induced and non-induced by lung cancer cells. (a) Induced. (b) Non-induced. Magnification: ×600. (B) The average expression of α-SMA in per cell was reflected by normalized fluorescent intensity. Data were shown as mean±SD of triplicate determinations. (C) Fluorescent analysis of apoptosis in fibroblasts induced and non-induced by lung cancer cells with PI and Hoechst after treatment with VP-16 (30 µM). Magnification: ×100. (D) The statistic analysis of percentage of apoptotic cells induced and non-induced by the lung cancer cells after treatment with different concentrations of VP-16 (0–60 µM). *p<0.05 compared with the control group. All the experiments were repeated at least three times.