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. 2013 Apr 16;8(4):e61754. doi: 10.1371/journal.pone.0061754

Figure 3. Analysis of GRP78 expression and effect of EGCG on VP-16 induced apoptosis in myofibroblasts.

Figure 3

(A) GRP78 protein assay by immunofluorescence imaging on (a) myofibroblasts and (b) fibroblasts. Magnification: ×600. (B) The average expression of GRP78 in per cell was reflected by normalized fluorescent intensity. Data were shown as mean±SD of triplicate determinations. (C) Fluorescent analysis of percentage of apoptotic cells for myofibroblasts by EGCG pretreated and non-EGCG pretreated groups after treatment with VP-16 (30 µM). Magnification: ×100. (D) The statistic analysis of percentage of apoptotic cells for myofibroblasts in EGCG pretreated and non-EGCG pretreated groups after treatment with different concentrations of VP-16 (0–60 µM). *p<0.05 compared with the control group. All the experiments were repeated at least three times.