Figure 5. FASN knockdown sensitizes both acquired and inherent drug resistant cells.

(A) Cells were treated with varying concentration of cerulenin for 24 h. Cells were washed and fresh medium was added for additional 48 h. MTT assay was performed and reading was taken at 570 nm using ELISA plate reader. (B) Hep3B cells, DRC and SCC were pretreated with 30 µM cerulenin. After 24 h treatment, medium was replaced with fresh medium with or without paclitaxel for 48 h and cell survival was evaluated by MTT assay. (C) DRC transfected with control or FASN siRNA for 24 h and western blotting was performed. (D) Hep3B cells, DRC and SCC were transfected with control and FASN siRNA as per manufacture instruction. Twenty-four hours posttransfection, medium with or without paclitaxel was added for 48 h and cell survival was evaluated by MTT assay.