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Huaier aqueous extract downregulates the Wnt/β-catenin pathway. (A) Expression of β-catenin and cyclin D1 was detected by western blot analysis in colorectal cancer cells either untreated or treated with Huaier extract for 48 h. (B) TOP/FOPflash reporter transiently infected colorectal cancer cells were treated with Huaier extract for 24 h. The Huaier extract decreased the percentage of TOP reporter. Bars denote the standard deviation (*P<0.05).
