Figure 1.

Detection of AR in UUTUC cells. (A) Western blot analysis of AR protein in BFTC 909 and BFTC 909 cells overexpressing AR. AR-positive human prostate cancer cells, LNCaP, were used as a control for AR expression. (B) Quantitative real-time PCR of AR mRNA expression in BFTC 909, BFTC 909 pBabeAR1 and BFTC 909 pBabeAR2. AR mRNA expression of BFTC 909 cells was set as 1. Results are expressed as log₁₀ of the relative amount of AR mRNA normalized to β-actin. Data are representative of three independent experiments. (C) XTT assay analysis of BFTC 909 pBabe, BFTC 909 pBabeAR1 and BFTC 909 pBabeAR2 cell growth. Cells were cultured for 2 or 4 days. AR, androgen receptor; UUTUC, upper urinary tract urothelial carcinomas.