Figure 2.

Cyclopamine affects breast cancer cell cycle and invasive ability. (A) Cell cycle distribution was evaluated by propidium iodide labeling and analysed by flow cytometry. (B) Cells treated with or without cyclopamine were trypsinized and transferred to the upper compartment of the modified Transwell chambers. Following 24 h of incubation, the invasive cells attached to the lower surface of the Matrigel-coated filter were fixed, stained and photographed under a phase contrast microscope and then counted in 15 randomly selected microscopic fields.