Figure 4.

U0126 partly rescues cyclin D1 inhibition to cyclopamine treatment. (A and B) Comparison of cyclin D1 expression between cells treated with or without cyclopamine. (C) Statistical analysis was conducted using a Student’s t-test. The expression levels are displayed as fold changes in band density. ^*P<0.01 vs. the control group. (D) Dose-dependent effects of cyclopamine on expression of estrogen receptor-α (ER-α) protein. MCF-7 cells were treated with indicated concentrations of cyclopamine for 24 h, and the level of ER-α was determined by western blot analysis.