Figure 4. Sequential treatment with ACM and imatinib did not affect the Fas receptor system.

(A) Cells were treated as described in Figure 2A. Cells were harvested and lysed, and the proteins were subjected to Western blot analysis using specific antibodies against Fas, Fas ligand (FasL) and procaspase-8. β-actin was used as loading control. (B) Cells were co-treated with AA and U0126 for 24 h. Western blot analyses of Fas and Fas ligand in ACM-IM-treated cells and AA+U0126-treated cells.