Table 1. Oligonucleotide primers used in the PCR amplification of various transcription factors.
Use | Gene | Primer Sequence |
cDNA cloning | dappuPNR | forward: 5′-AGTATCCCAACGGAGTGACG-3′ |
reverse: 5′-TACTGAGGATCCCGGGTCA-3′ | ||
cDNA cloning | dappuDSF | forward: 5′-CATCGTCTCCCCTCCTTGTA-3′ |
reverse: 5′-GGGGGAAAGGAAATCTCATC-3′ | ||
cDNA cloning | dappu & dapmag Met | forward: 5′-CCTTACGGAAAGCATCTTTAGTG-3′ |
reverse: 5′-CGTATGAATTAAAACAGCTTATTAGAAGTC-3′ | ||
Reporter Assay | GAL4 | forward: 5′- TATT ACTAGTGGCATGAAGCTACTGTCTTCTATCGAACAAG-3′ |
reverse: 5′- AATT TTCGAATCTAGATGATATCAACGCGTCAAGTCGAC-3′ | ||
Reporter Assay | dappuPNR | forward: 5′-TACTAT GAATTCCGACCGGAAATTCTGGCCGAA-3′ |
reverse: 5′-TACTAT TTCGAATTAATTTTTGTACATATCGCAGAG-3′ | ||
Reporter Assay | dappuDSF | forward: 5′-CAAC GAATTCAACAGCGTCCATCACCATTTC-3′ |
reverse: 5′-CTCT TTCGAACATCGATGAAACCAAACCAA-3′ | ||
Reporter Assay | dappuMet | forward: 5′-TACTAT GAATTCATACATCAGAATGTGGATTTACGGGT-3′ |
reverse: 5′-TACTAT ACGCGTTCACGGACTACTAGTTCCAG-3′ |
Bold denotes added restriction sites and italics denote spacer nucleotides added to facilitate proper cutting of the sequence. Some primers used in the reporter assay constructs were situated upstream or downstream of the sequence targeted for amplification.