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. 2013 Apr 17;8(4):e61715. doi: 10.1371/journal.pone.0061715

Table 1. Oligonucleotide primers used in the PCR amplification of various transcription factors.

Use Gene Primer Sequence
cDNA cloning dappuPNR forward: 5′-AGTATCCCAACGGAGTGACG-3′
reverse: 5′-TACTGAGGATCCCGGGTCA-3′
cDNA cloning dappuDSF forward: 5′-CATCGTCTCCCCTCCTTGTA-3′
reverse: 5′-GGGGGAAAGGAAATCTCATC-3′
cDNA cloning dappu & dapmag Met forward: 5′-CCTTACGGAAAGCATCTTTAGTG-3′
reverse: 5′-CGTATGAATTAAAACAGCTTATTAGAAGTC-3′
Reporter Assay GAL4 forward: 5′- TATT ACTAGTGGCATGAAGCTACTGTCTTCTATCGAACAAG-3′
reverse: 5′- AATT TTCGAATCTAGATGATATCAACGCGTCAAGTCGAC-3′
Reporter Assay dappuPNR forward: 5′-TACTAT GAATTCCGACCGGAAATTCTGGCCGAA-3′
reverse: 5′-TACTAT TTCGAATTAATTTTTGTACATATCGCAGAG-3′
Reporter Assay dappuDSF forward: 5′-CAAC GAATTCAACAGCGTCCATCACCATTTC-3′
reverse: 5′-CTCT TTCGAACATCGATGAAACCAAACCAA-3′
Reporter Assay dappuMet forward: 5′-TACTAT GAATTCATACATCAGAATGTGGATTTACGGGT-3′
reverse: 5′-TACTAT ACGCGTTCACGGACTACTAGTTCCAG-3′

Bold denotes added restriction sites and italics denote spacer nucleotides added to facilitate proper cutting of the sequence. Some primers used in the reporter assay constructs were situated upstream or downstream of the sequence targeted for amplification.