Figure 3. In vitro target assay for microRNAs of the DNMT3A transcript.

[A] Diagram of miR-1741 binding site in the DNMT3A 3′-UTR. [B] Expression vector map for eGFP with DNMT3A 3′-UTR and Ds-Red with miR-1741. [C and D] After co-transfection of pcDNA-eGFP-3′-UTR for the DNMT3A transcript and pcDNA-DsRed-miRNA for the miR-1741, the fluorescence signals of GFP and DsRed were detected using FACS [C] and fluorescent microscopy [D].