Figure 4. In vitro target assay of microRNAs on the DNMT3B transcript.

[A] Diagram of miR-16c, miR-222, and miR-1632 binding sites in the DNMT3B 3′-UTR. [B] Expression vector map for eGFP with DNMT3B 3′-UTR and Ds-Red with each miRNA. [C and D] After co-transfection of pcDNA-eGFP-3′-UTR for the DNMT3B transcript and pcDNA-DsRed-miRNA for the miR-16c, miR-222, and miR-1632, the fluorescence signals of GFP and DsRed were detected using FACS [C] and fluorescent microscopy [D].