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. 2013 Apr 17;8(4):e61851. doi: 10.1371/journal.pone.0061851

Table 3. Herbicides toxicity effects on the PSII electron transport efficiency (1-VJ) of IL and selected D1 random mutants, and corresponding R/S values.

Strains Atrazine Terbuthylazine Linuron
I50×10−7 M R/S I50×10−7 M R/S I50×10−7 M R/S
(±SE) (±SE) (±SE)
IL 2.4±0.11 / 1.0±0.08 / 1.1±0.05 /
P162S/F211S 19.7±1.57 8.2 5.9±0.26 6.2 2.0±0.17 1.9
M172L 2.2±0.03* 0.9 1.1±0.02* 1.1 1.1±0.06* 1.0
L200I 1.8±0.06 0.7 0.6±0.04 0.6 0.9±0.06 0.8
G207S 2.1±0.09* 0.9 4.3±0.18 4.4 1.3±0.09 1.2
I281T 2.0±0.07 0.8 0.7±0.02 0.8 1.3±0.05 1.2

The I50 is the molar herbicide concentration, which induces 50% inhibition of the parameter 1-VJ. The R/S were calculated as ratios of mutant I50 and I50 of the reference strain; the asterisks indicate values that do not differ significantly from the IL strain at p≤0.05 (Mann-Whitney U Test). We considered that R/S<0.9 values define increased herbicide sensitivity, R/S>1.1 – increased herbicide resistance and 0.9≤R/S≤1.1 – no significant alterations in the mutants herbicide sensitivity relative to the reference strain.