Table 3. Herbicides toxicity effects on the PSII electron transport efficiency (1-VJ) of IL and selected D1 random mutants, and corresponding R/S values.
Strains | Atrazine | Terbuthylazine | Linuron | |||
I50×10−7 M | R/S | I50×10−7 M | R/S | I50×10−7 M | R/S | |
(±SE) | (±SE) | (±SE) | ||||
IL | 2.4±0.11 | / | 1.0±0.08 | / | 1.1±0.05 | / |
P162S/F211S | 19.7±1.57 | 8.2 | 5.9±0.26 | 6.2 | 2.0±0.17 | 1.9 |
M172L | 2.2±0.03* | 0.9 | 1.1±0.02* | 1.1 | 1.1±0.06* | 1.0 |
L200I | 1.8±0.06 | 0.7 | 0.6±0.04 | 0.6 | 0.9±0.06 | 0.8 |
G207S | 2.1±0.09* | 0.9 | 4.3±0.18 | 4.4 | 1.3±0.09 | 1.2 |
I281T | 2.0±0.07 | 0.8 | 0.7±0.02 | 0.8 | 1.3±0.05 | 1.2 |
The I50 is the molar herbicide concentration, which induces 50% inhibition of the parameter 1-VJ. The R/S were calculated as ratios of mutant I50 and I50 of the reference strain; the asterisks indicate values that do not differ significantly from the IL strain at p≤0.05 (Mann-Whitney U Test). We considered that R/S<0.9 values define increased herbicide sensitivity, R/S>1.1 – increased herbicide resistance and 0.9≤R/S≤1.1 – no significant alterations in the mutants herbicide sensitivity relative to the reference strain.