. 2013 Apr 17;8(4):e62126. doi: 10.1371/journal.pone.0062126

Table 1. Primers for the multiplex tetra-primer ARMS-PCR for SNPs associated with breast and gynecologic cancers.

SNP	Primer	Primer Sequence	Working concentration(nM)	Length (bp)
rs4784227	Forward inner primer(T):	AGGTGACACTATAGAATAAAAAGTCCCAATTTGTAGTGTTTGaT^a ^, ^b ^, ^c	40	322
	rs4784227-476T-2A
	Reverse inner primer(C):	GTACGACTCACTATAGGGAATGGGAGTATTTACATCACAATAATgG^a ^, ^b ^, ^c	40	297
	rs4784227-527G-2G
	Forward outer primer:	AGGTGACACTATAGAATACTGACCCCTTTAGACACGG^a	6.5
	rs4784227-268
	Reverse outer primer:	GTACGACTCACTATAGGGAGGGCTTCAACACAGTCAGTTC^a	6.5
	rs4784227-760
rs1219648	Forward inner primer(G):	AGGTGACACTATAGAATACACGCCTATTTTACTTGACACAaG^a ^, ^b ^, ^c	45	269
	rs1219648-278G-2A
	Reverse inner primer(A):	GTACGACTCACTATAGGGAAGCCATGGCCATCCTTGAAGAaT^a ^, ^b ^, ^c	40	199
	rs1219648-323T-2A
	Forward outer primer:	AGGTGACACTATAGAATACACAATGGCGCAGAATTA^a	6.5
	rs1219648-162
	Reverse outer primer:	GTACGACTCACTATAGGGACTGGACAGGTCATTGTGGTG^a	7
	rs1219648-509
rs3803662	Forward inner primer(C):	AGGTGACACTATAGAATACTCTCCTTAATGCCTCTATAGCTGTaC^a ^, ^b ^, ^c	45	259
	rs3803662-275C-2A
	Reverse inner primer(T):	GTACGACTCACTATAGGGACCACAGTTTTATTCTTCGCTAAcA^a ^, ^b ^, ^c	80	357
	rs3803662-324A-2C
	Forward outer primer:	AGGTGACACTATAGAATAGTCCTTGGCTGTTCTGTGA^a	11.5
	rs3803662-5
	Reverse outer primer:	GTACGACTCACTATAGGGATCCCCAAGGAGACAAAGGTA^a	7
	rs3803662-496
rs889312	Forward inner primer(A):	AGGTGACACTATAGAATATGCCCCTGCTGGAGAAAGtA^a ^, ^b ^, ^c	60	213
	rs889312-382A-2T
	Reverse inner primer(C):	GTACGACTCACTATAGGGATGATTTGTAGTCTCTTAATTTGCACAaG^a ^, ^b ^, ^c	75	340
	rs889312-428G-2A
	Forward outer primer:	AGGTGACACTATAGAATACCTGGGTCCTTAGCATTCC^a	11
	rs889312-126
	Reverse outer primer:	GTACGACTCACTATAGGGAATCTGTGCCCTGAAGTGAGTAG^a	8.5
	rs889312-557
rs750749	Forward inner primer(C):	AGGTGACACTATAGAATAGAGGCTGAGAACTAATAATAATTTTcC^a ^, ^b ^, ^c	50	371
	rs750749-275C-2C
	Reverse inner primer(T):	GTACGACTCACTATAGGGATCAGATGAAAACTGCTGTGTAAGaA^a ^, ^b ^, ^c	75	224
	rs750749-325A-2A
	Forward outer primer:	AGGTGACACTATAGAATACCCACAGAGTTGGAATGCTT^a	11
	rs750749-139
	Reverse outer primer:	GTACGACTCACTATAGGGATGCATAAAGTGGGTCCTGC^a	7.5
	rs750749-608
rs749292	Forward inner primer(G):	AGGTGACACTATAGAATACCTTCTTCAAACCTCGGAGTaG^a ^, ^b ^, ^c	35	282
	rs749292-429G-2A
	Reverse inner primer(A):	GTACGACTCACTATAGGGAGATAGAAATTGTGCAGGAATCCaT^a ^, ^b ^, ^c	25	330
	rs749292-472T-2A
	Forward outer primer:	AGGTGACACTATAGAATATCCACGGACAGAGCAGG^a	4.5
	rs749292-180
	Reverse outer primer:	GTACGACTCACTATAGGGAGATTAGGGGACCTCCGTGA^a	6
	rs749292-673
Tag-F		AGGTGACACTATAGAATA^d	500
Tag-R		GTACGACTCACTATAGGGA^d	500

Underlined is the universal tag sequence at the 5′ end of the chimeric primers.

Allele-specific nucleotides at the 3′ end are indicated in bold letters.

Specificity is increased by the introduction of a deliberate mismatch at position -1 of the polymorphism site, indicated by bold lower case letters.

A pair of universal primers annealing to the 5′ portion of each chimeric primer.