Figure 3. Effect of EE on HIV-infection of CD4⁺ T-cells and macrophages.

A) Released p24 levels in the culture media after 7 days of infection when CD4⁺ T-cells where pre-treated with EE (pre EE), treated with EE before and after infection (prepost EE) or only after infection (post EE). Bars represent mean ± SEM from 4 independent experiments with different donors. B) Released p24 levels in the culture media after 7 days of infection when macrophages where pre-treated with EE (pre EE), treated with EE before and after infection (prepost EE) or only after infection (post EE). Bars represent mean ± SEM from 4 independent experiments with different donors. **P<0.01. C) Comparison between CD4⁺ T-cells and macrophages from the same donors pre-treated with E₂ and EE. Dot plots represent percent of p24+ cells. D) Percent of suppression of HIV-infection respect to infected control in macrophages from the same donors pre-treated with E₂ (black dots) or EE (grey dots) in parallel. Each dot represents one individual.