Figure 4. Dose response effect of E₂ on HIV-infection.

A) Released p24 levels in the culture media after 7 days of infection when CD4⁺ T-cells where pre-treated with indicated doses of E₂. Bars represent mean ± SEM from triplicates. Representative of 3 independent experiments with different donors. *P<0.05 **P<0.01. B) Released p24 levels in the culture media after 7 days of infection when macrophages where pre-treated with indicated doses of E₂ (black bars) or EE (grey bars). Bars represent mean ± SEM from triplicates. Representative of 3 independent experiments with different donors. *P<0.05 **P<0.01. C) Percent of GFP positive cells after infection with HIV-GFP at MOI 1 or MOI 0.1 in CD4⁺ T-cells pre-treated with E₂ (pre E₂) or treated before and after infection (prepost E₂).