Figure 5. The effect of 1% methanol and 1% ethanol on Colletotrichum gloeosporioides ES026 fermentation in a time course study.

Colletotrichum gloeosporioides ES026 was cultured in liquid growth medium consisting of potato 200 g/L (soup extract), sucrose 10 g/L and 1% methanol or 1% ethanol, with a blank control (Ctr). The culture was kept shaking at 150 rpm at 25°C for the indicated incubation time. The mycelium biomass was then compared among the treatment groups (A). The HupA yield with ethanol (B) and methanol (C) treatment was analyzed by HPLC of which, Curve 1 depicts authentic HupA (2.0 µg·ml⁻¹), while curves 2 to 7 represent the HupA from 3 to 8 days of fermentation, respectively and the yield was quantified (D). The arrows indicate the peak for HupA. Each data point is the average of three replicates with error bar representing standard deviation.