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. 2013 Apr 17;8(4):e61506. doi: 10.1371/journal.pone.0061506

Figure 3. Effect of Thy-1 on lamellipodia formation, and endothelial cell viability and adhesion.

Figure 3

(a) Over-expression of Thy-1 inhibited the formation of lamellipodia in HUVEC. HUVEC were tranfected with pcDNA3.1 (control) or Thy-1 pcDNA3.1. At 45 h after transfection, phalloidin staining of HUVEC was then performed as described in the Materials and methods. Scale bar, 25 mm. Triangles indicated the lamellipodia. (b) Over-expression of Thy-1 did not affect the viability of HUVEC. The adhesion of Thy-1-transfected HUVEC to collagen was decreased as compared with vector-transfected HUVEC examined by MTT assay (c) or by cell count (d). (e) This effect was also observed in the HMEC-1 transfected with Thy-1 pcDNA3.1. Values represent the means±s.e.mean. (n = 4–6). *p<0.05.