Figure 4. Identification of Nudc and TFII-I as potential Akt binding partners.

Representative MS/MS spectra of Nudc (a) and TFII-I (b) peptides identified from the co-IP products. The sequence of peptides was assigned with a single letter abbreviation based on the fragment ions observed for the peptide segments. N-terminal b ions and C-terminal y ions resulting from the amide bond cleavage are labeled. * denotes the addition of 145.0198 Dalton due DSP-crosslinking. Western blotting indicated that Nudc co-immunoprecipitated with Akt in Neuro 2A cells (a). nc, negative control using IgG instead of Akt antibody at a similar protein level.