Figure 9. Recall memory response results in an enhanced killing of F. tularensis SchuS4.

The BMDMs were infected with F. tularensis SchuS4 and co-incubated with the splenocytes from naïve, FTL_0325 or FTL_0304 immunized mice. The BMDMs were lysed 72 hrs PI, diluted 10-fold and plated on MH-chocolate agar plates to determine the intracellular bacterial replication. The results were expressed as log₁₀ CFU/ml and are representative of two independent experiments. The data were analyzed using ANOVA with Tukey-Kramer Multiple Comparison post-test and P values were recorded. ***P<0.001.