Figure 1.

Transient transfection of Dicer small interfering RNA (siDicer) and effects of Dicer depletion on cell viability, proliferation and the cell cycle in ovarian cancer cells. (A) Expression of Dicer mRNA is detected by quantitative polymerase chain reaction (qPCR). The results are normalized to the expression of the reference gene β-actin. (B) Western blot analysis demonstrates a downregulation of Dicer protein in siDicer-A2780 cells. (C) Cell viability as evaluated in vitro by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) proliferation assays each day for 5 days following transfection. All data are representative of three independent experiments. (D) Cell proliferation as measured by a BrdU incorporation assay. The results are expressed as the fold change relative to the corresponding siNC-A2780/-SKOV3 control. Bars, standard deviation. (E) Cell cycle as analyzed by a propidium iodide flow cytometry assay. The percentages of S, G2/M and G0/G1 phase cells are calculated from three independent experiments. Bars, standard deviation.