Figure 4.

Loss of EZH2 increases Dicer expression in A2780 cells. (A) Western blot analysis shows downregulation of EZH2 protein in shEZH2-A2780 cells. (B) Quantitative real time-polymerase chain reaction (qRT-PCR). Dicer mRNA expression is elevated in short hairpin (sh) EZH2-A2780 cells compared with untransfected A2780 cells and shVector-A2780 cells. All data are representative of three independent experiments and normalized to β-actin. (C) Dicer protein is overexpressed in shEZH2-A2780 cells compared with untransfected A2780 and shVector-A2780 cells. β-actin, internal control. Bottom: representative western blot analysis of Dicer; top: histogram of quantified results of western blot analysis. All data are representative of three independent experiments.